论文模板

干旱胁迫下罗浮槭苗期的生理和形态变化
季 华1,傅 强1,姜德伟1,2,邵 莉1 ,陈龙清1*

(1.华中农业大学 园艺林学学院、园艺植物生物学教育部重点实验室,湖北 武汉 430070;2.广州普邦园林股份有限公司,广东 广州 510600)
 

摘要:以罗浮槭1年生幼苗为材料,测定其在不同干旱处理下的生理和形态变化,以及利用扫描电镜观察不同处理下的叶片生理指标和气孔变化,结果表明:随着处理时间的延长,株高降低;幼苗的MDA含量、可溶性蛋白含量、SOD活性和POD活性均高于对照,前三者均在干旱处理10 d时达到最高值,POD活性在干旱处理20 d时最高,而Pro含量低于对照。MDA含量和POD活性呈先增加后减少的趋势,处理组的Pro含量和可溶性蛋白含量变化不大。此外,在干旱处理下罗浮槭的叶片蜡质增多,总气孔密度减少,气孔器的长、宽和开张度变化不明显。

关键词:罗浮槭;干旱胁迫;生理响应;气孔
中图分类号:S792.35     文献标识码:A   文章编号:1001-8581(2012)01-0001-04


Physiological and Morphological Changes of Acer fabri Seedlings under Drought Stress

JI Hua1, FU Qiang1, JIANG De-wei1,2, SHAO Li1, CHEN Long-qing1*
(1. College of Horticulture and Forestry, Huazhong Agricultural University; Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan 430070, China; 2. Guangzhou Pubang Landscape Limited Company, Guangzhou 510600, China)
Abstract: Using one-year-old seedlings of Acer fabri as the tested materials, the physiological and morphological changes in different drought treatments were tested, and the changes in the physiological indexes and stomata of leaves were investigated by SEM. The results showed that the plant height decreased with the increase of treatment time. The content of melondialdehyde (MDA) and soluble protein, and the activity of superoxide dismutase (SOD) and peroxidase (POD) in seedlings in drought treatments were higher than those in the check, the three former reached the highest on the 10th day after drought stress, and the latter was the highest on the 20th day after drought stress. But the content of proline (Pro) in drought treatments was lower than that in the check. Under drought stress, the content of MDA and activity of POD firstly increased and then decreased, the content of Pro and soluble protein changed little, the leaf waxiness increased, total stomatic density decreased, while the length, width and open degree of stomata had no obvious changes.

Key words: Acer fabri; Drought stress; Physiological response; Stomata

收稿日期:2021-11-22

基金项目:中央高校基本科研业务费专项资金(2010QC028);华中农业大学硕士研究生创新研究项目(3003-457)。
作者简介:季华(1974─),女,讲师,在读博士,主要从事园林植物种质资源的收集与抗旱研究。*通讯作者:陈龙清。
 

    罗浮槭(Acer fabri)是槭树科槭属的常绿小乔木,因其鲜红色的翅果集生于树冠外,又名红翅槭。其树形优美、枝叶茂密,新发嫩枝、嫩叶呈暗红色或绛紫色,因而观赏价值高。同时具有较强的耐寒、耐荫能力[1],较适合作为林冠的第二层配置,也可用作风景林、生态林和四旁绿化树种。

     我国槭属植物资源丰富,占世界槭属种类的50%以上[2],但大多数栽培观赏品种源于日本、欧洲和北美地区,而对国内槭属植物的开发利用不够,特别是常绿的槭树类[3]。水是植物体的组成成分,而目前水资源匮乏已成为限制植物应用的重要因子之一。虽然对槭树类形态学、细胞学、繁殖特性等已有较多研究[4-5],但对槭树类的抗旱研究较少。本试验探讨了干旱胁迫下罗浮槭幼苗的生理和形态变化,以期为我国城市槭属树种的引种提供科学依据和节约型园林提供新的观赏植物资源。

1材料与方法

…………

…………

参考文献:
[1] 中国科学院中国植物志编辑委员会.中国植物志(46卷)[M].北京:科学出版社,1981:211.

[2] 徐廷志.云南槭树红叶观赏植物资源开发利用[J].中国野生植物资源,1989,9(3):10-12.